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NR. 1 (I)/2007
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Niestabilność
mikrosatelitarna w zmianach nowotworowych powiek
Microsatellite Instability in Malignant Eyelids Lesions
Renata Zalewska1, Ewa
Proniewska-Skrętek1, Zofia Mariak1, Witold
Pepiński2, Małgorzata Skawrońska2, Jerzy
Janica2
1Klinika Okulistyki Akademii Medycznej w Białymstoku
Kierownik: prof. dr hab. n. med. Zofia Mariak
2Zakład Medycyny Sądowej Akademii Medycznej w
Białymstoku
Kierownik: prof. dr hab. n. med. Jerzy Janica |
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| Summary: |
Analysis of microsatellite
instability (MSI) using specific genetic markers is the
method of choice in suppressor genes localization
assessment responsible for malignant transformation.
The objective of the study was assessment of MSI in
eyelid skin tumours.
Material and methods: The studied group comprised 24
patients (12 females and 12 males) aged 43-81 years (average
69.2±9.6) after eyelid tumors surgical resection. Tumor
samples had histopathological evaluation using the MSI
Analysis System (Promega) containing five
fluorescenty-labeled mononucleotide repeat markers:
BAT-25, BAT-26, NR-21, NR-24, MONO-27 and two
pentanucleotide repeat markers Penta C and Penta D.
Results: Histopathological assessment revealed
basocellular carcinoma (4 cases), spinocellular carcioma
(2 cases), planoepithelial papilloma (14 cases),
keratopapilloma (3 cases), polypus fibrous polyp (1 case).
MSI was found only at NR-24 in planoepithelial papilloma
in four males aged 43-69 years.
Conclusions: The obtained results suggest limited
efficacy of the MSI Analysis System in eyelids malignant
lesions identification. Further studies with larger
number of patients are needed to elucitade contribution
of MSI to development of eyelid skin tumours. |
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| Key words: |
microsatellite instability
(MSI), malignant eyelids lesions. |
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