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NR 1-2/2004
SUPLEMENT
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Ekspresja białek
Bcl-2, Bcl-xl, Bak i Bax w aksonach nerwu wzrokowego u chorych z
jaskrą dokonaną
The expression of Bcl-2,
Bcl-xl, Bak and Bax proteins in axons of the optic nerve in
absolute glaucoma
Renata Zalewska1, Joanna
Reszeć2, Zofia Mariak1, Stanisław
Sulkowski2
1 Z Kliniki Okulistyki Akademii Medycznej w
Białymstoku
Kierownik: dr hab. n. med. Zofia Mariak
2 Zakład Patomorfologii Akademii Medycznej w
Białymstoku
Kierownik: prof. dr hab. n. med. Lech Zimnoch |
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| Summary: |
Purpose: The aim of
our study was the evaluation of Bcl-2, Bcl-xl, Bak and
Bax immunoexpression in axons of the optic nerve with
absolute glaucoma.
Material and methods: This study was conducted on
19 patients with absolute glaucoma, suffered from severe
ophthalmalgia, treated of necessity by enucleation in
1991-2003 in our Department of Ophthalmology. The
samples were immuno-stained with antibodies for Bcl-2,
Bak, Bax and Bcl-xl protein. The reactions were
performed in Labelled Streptavidin Avidin Biotin (LSAB)
technique. The evaluation of immuno-staining was
calculated as the percentage of cells with positive
reaction to the total number of cells encountered in 10
representative fields, by light microscopy using a x 20
objective lens. Scores were based on the following scale:
(++), over 50% of the field showing positive
immuno-staining, (+) 10-50% with positive
immuno-staining.
Results: In our study proapoptotic Bak and Bax
protein expression was stronger than antiapoptotic
Bcl-2, Bcl-xl protein expression; Bak/Bcl-2 (p = 0,008),
Bax/Bcl-2 (p = 0,0007), Bak/Bcl-xl (p = 0,0356), Bax/Bcl-xl
(p = 0,0077).
Conclusions: The results of our study demonstrate
high proapoptotic trends in axons of the optic nerve in
the stage of absolute glaucoma. |
| Słowa kluczowe: |
aksony, nerw wzrokowy,
jaskra, apoptoza, Bcl-2, Bcl-xl, Bax, Bak. |
| Key words: |
axons, the optic nerve,
glaucoma, apoptosis, Bcl-2, Bcl-xl, Bax, Bak. |
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