|
NR 3/2004
SUPLEMENT
|
|
|
|
|
|
|
|
|
Inhibitory proteaz w
surowicach pacjentów z cukrzycow± retinopati± prost±
Proteinases inhibitors in
sera of diabetic patients with non-proliferative retinopathy
Piotr Skopiński1,2, Barbara
Duda-Król3, Anna Lipińska4, Ewa Sommer5,
Adamina Borowska5, Małgorzata Filewska5,
Jerzy Szaflik1, Ewa Skopińska-Różewska5,
Ewa Struzik1
1Z Katedry i Kliniki Okulistyki II Wydziału
Lekarskiego Akademii Medycznej w Warszawie
Kierownik: prof. dr hab. n. med. Jerzy Szaflik
2Z Zakładu Histologii i Embriologii, Centrum
Biostruktury
Kierownik: prof. dr hab. n. med. Stanisław Moskalewski
3Z III Kliniki Chorób Wewnętrznych II Wydziału
Lekarskiego Akademii Medycznej w Warszawie
Kierownik: prof. dr hab. n. med. Wojciech Łada
4Z Kliniki Chorób Wewnętrznych Oddziału Stomatologii
Akademii Medycznej w Warszawie
Kierownik: prof. dr hab. n. med. Danuta Pfeiffer
5Z Zakładu Diagnostyki Laboratoryjnej i Immunologii
Instytutu GruĽlicy i Chorób Płuc w Warszawie
Kierownik: prof. dr hab. n. med. Ewa Skopińska-Różewska |
|
|
| Summary: |
Diabetes is associated
with a hypercoagulable state. Eighty percent of patients
with diabetes mellitus die due to various thrombotic
vascular complications. Disorder of coagulation and
fibrynolysis is associated with diabetic retinopathy and
nephropathy (1, 2, 3). Angiogenesis requires degradation
of vascular basement membrane prior to migration and
proliferation of endothelial cells. Various serine
proteases play important role in this process.
The homeostatic system is also the source of endogenous
inhibitors of angiogenesis (4, 5, 6,). Human serum
contains various factors able to induce or suppress
formation of new blood vessels. The aim of the present
study was to evaluate the activity of some angiogenesis
inhibitors, anti-proteases, anti-thrombin III, a1
anti-trypsin and a2 anti- plasmin in sera of patients
with diabetes mellitus type 1 and 2 and
non-proliferative retinopathy, and to correlate this
activity to total angiogenic potential of these sera,
measured by mice cutaneous test. Sera of 22 persons with
DM1, aged 33-70 years, 35 persons with DM2, aged 37- 79
years, and 51 healthy people, aged 22-80 years (as
control group) were studied. Direct serum-induced
cutaneous angiogenesis test in mice (SIA) was applied
(7, 8). Berichrom (ade Behring) tests and
immunoturbidimetric method were used for evaluation of
anti-proteases activity. Angiogenic activity of DM1
patients sera was statistically lower than this
parameter in DM2 patients and in control group. Levels
of anti-proteases were similar in DM1, DM2 and control
group, with one exception: anti-thrombin level was lower
in DM2 patients' sera than this in the control group.
Analysis of correlation revealed important difference in
behaviour of DM1 sera, as compared to other groups.
Significant negative correlation was observed between
angiogenic activity and anti-thrombin, as well as
anti-trypsin level of DM1 patients' sera. On the other
hand, correlation analysis performed for the sera of
control group revealed significant positive correlation
between their angiogenic activity and anti-thrombin
level. No other correlations were found. |
| Słowa kluczowe: |
antytrombina III,
α1-anty-trypsyna, α2-anty-plazmina, angiogeneza,
retinopatia prosta, cukrzyca typu 1 (DM1) i typu 2
(DM2). |
| Key words: |
antithrombin III,
α1-antitrypsin, α2-antiplasmin, angiogenesis,
non-proliferative retinopathy, diabetes type 1(DM1) and
type 2(DM2). |
|
|
|
|
|
|
