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NR 3/2004
SUPLEMENT
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Badania
histopatologiczne w eksperymentalnym zapaleniu błony naczyniowej
Histopathologic studies in
experimental uveitis
Marta Misiuk-Hojło1, Zdzisław
Woźniak2, Stanisław Szymaniec3, Czesław
Ługowski4, Karolina Agopsowicz1
1Z Kliniki Okulistyki Akademii Medycznej we Wrocławiu
Kierownik: prof. dr hab. n. med. Maria Hanna Niżankowska
2Z Zakładu Anatomii Patologicznej Akademii Medycznej
we Wrocławiu
Kierownik: prof. dr hab. n. med. Jerzy Rabczyński
3Z Zakładu Terapii Doświadczalnej Polskiej Akademii
Nauk we Wrocławiu
Kierownik: prof. dr hab. n. med. Stanisław Szymaniec
4Z Zakładu Mikrobiologii Polskiej Akademii Nauk we
Wrocławiu
Kierownik: prof. dr hab. Czesław Ługowski |
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| Summary: |
Experimental uveitis is
one of the main models in the diseases of
autoimmunological background. The purpose of this paper
was to analyze the quantitative histological changes in
the experimental uveitis, induced by different types of
homogenous endotoxin salts of Havnia alvei. We studied
74 eyes of Lewis rats (males) divided into 4 groups.
Each group received a homogenous salt of Havnia alvei in
a single subcutaneous injection. In the 1 group-LPS
Ca++, in 2 group-LPS Na++, in 3 group-LPS 981, in 4
group physiological salt (control group). The histologic
and immunocitochemical examinations were performed after
24, 48 hours, and after 4 and 7 days following the
injection. The histologic changes were analyzed (he
intensity of inflammatory reaction) using a Highly
Optimazed Microscope Enviroment system. The most
intensive inflammation was observed in experimental
group after 24 hours (n the LPS 981D group in 5 rats out
of 6, in LPS Ca++ group in 3 rats out of 6). After 48
hours the intensity of inflammatory reaction visibly
decreased. On the fourth day the inflammation revealed a
minimal intensity and after 7 days was practically
absent. In the control group minimal inflammation was
observed only in a few rats. Cilliary body hypermia was
present for 48 hours in most of the experimental rats.
Only a few of them had hyperemia on the fourth day. In
the posterior segment minimal inflammation was noted at
the end of the first day (I and II – nd group after 24
hours); this process continued until the fourth day, on
the 7th day disappeared.
Conclusions: The most intensive inflammation of
the anterior and posterior choroidal segment is caused
by homogenous salts of Havnia alvei 981. |
| Słowa kluczowe: |
doświadczalne zapalenie
błony naczyniowej, endotoksyna Havnia alvei, badania
histopatologiczne. |
| Key words: |
experimental uveitis,
Havnia alvei endotoxin, histopathologic study. |
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