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NR 3/2004
SUPLEMENT
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Ocena
przeciwdrobnoustrojowego działania oleju silikonowego – badania
in vitro
In vitro studies on
antimicrobial properties of silicon oil
Jerzy Mackiewicz1, Maria
Kozioł-Montewka2, Ewa Kosior-Jarecka2,
Agnieszka Szczepanik2, Małgorzata Wójtowicz2,
Zbigniew Zagórski1
1Z Katedry i I Kliniki Okulistyki Akademii Medycznej
im. prof. Feliksa Skubiszewskiego w Lublinie
Kierownik: prof. dr hab. n. med. Zbigniew Zagórski
2Z Katedry i Zakładu Mikrobiologii Lekarskiej
Akademii Medycznej im. prof. Feliksa Skubiszewskiego w Lublinie
Kierownik: prof. dr hab. n. med. Maria Kozioł-Montewka |
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| Summary: |
Purpose: The aim of
the study was to evaluate antimicrobial properties of
silicon oil in vitro against Staphylococcus aureus,
Pseudomonas aeruginosa, Candida albicans, which are
considered the major causative agents of endophthalmitis.
Material and methods: The clinical isolates of
representative microorganisms (S. aureus, P. aeruginosa,
C. albicans) were selected. The bacteria and the fungus
were separately inoculated in PDMS 5000 (produced by
AcriMed, Germany). Control inoculations in physiological
saline and sugar bouillon were performed. The samples of
0.01 ml from each medium were diluted, according to
serial dilution procedure and inoculate on Petri plate
dishes 5% sheep blood agar for bacteria and Sabouraud
medium for Candida albicans. After 24 h incubations for
bacteria and 48 h incubations for fungus, CFUs were
counted.
Results: All the microorganisms revealed an
apparent decrease in CFUs in PDMS 5000. The total
elimination was observed for S. aureus after 5 days. For
P. aerugisosa solitary colonies (less than 25 CFUs) were
observed up to 7 days. After 7 days of incubation no
growth of P. aeruginosa was observed. High C. albicans
CFU values were counted up to 3 day of the incubation.
After 5 days single fungal colonies were observed. CFUs
of the examined microorganisms declined slightly in
physiologic saline. A growth pattern similar to the
growth curve of microorganisms was observed in sugar
boullion.
Conclusions: Our study indicates that silicon oil
could have an antimicrobial activity against
Staphylococcus aureus, Pseudomonas aeruginosa, Candida
albicans, which are considered the major causative
agents of postoperative endophthalmitis. |
| Słowa kluczowe: |
zapalenie wnętrza gałki,
olej silikonowy, badania in vitro. |
| Key words: |
endophthalmitis, silicon
oil, in vitro studies. |
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