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NR 10-12/2005
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Przeciwciała
przeciwsiatkówkowe (PPS) w surowicy chorych z czerniakiem
naczyniówki – doniesienie wstępne
Serum antiretinal antibodies
(ARA) in patients with choroidal melanoma – preliminary report
Agnieszka Kubicka-Trząska1,
Izabella Karska-Basta1, Katarzyna Krukierek-Małec2
1Z Katedry i Kliniki Okulistyki Collegium Medicum
Uniwersytetu Jagiellońskiego w Krakowie
Kierownik: prof. dr hab. n. med. Maria Starzycka
2Z Pracowni Cytoimmunologii Zakładu Biochemii
Klinicznej II Katedry Chorób Wewnętrznych
Collegium Medicum Uniwersytetu Jagiellońskiego w Krakowie
Kierownik: prof. dr hab. n. med. Andrzej Szczeklik |
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| Summary: |
Purpose: To
estimate serum antiretinal antibodies (ARA) in patients
with choroidal melanoma after therapy in 3 months
follow-up period.
Material and methods: 24 patients at the age 37 –
72 years (mean: 57.8 years) with choroidal melanoma were
examined. In all cases routine ophthalmic examination
with A- and B-scan ultrasonography and fluorescein
angiography were performed. In some subjects also
indocyanine green angiography was performed.
Ruthenium-106 plaques were used in 16 cases, in 5 cases
treatment with Iodine-125 plaques was performed. Three
patients underwent enucleation. In all cases ARA were
determined in a serum dilution 1:10 by indirect
immunofluorescence test on normal monkey retina as a
substrate and FITC-labeled anti-human IgA, IgG, IgM
serum (Euroimmun – Germany). ARA were scored before and
within 3 months, after the therapy was performed.
Results: Before treatment ARA were present in 15
patients (62.5%) with choroidal melanoma in serum
dilution 1:10. In a control group ARA were present in 3
cases (12.5%) in serum dilution 1:10. After therapy
within 3 months follow-up period ARA occurred in serum
of further 7 patients, all treated with Ruthenium-106
plaques. Fluorescence of outer retinal segments was
present in 7 cases, while serum of remaining 15 patients
demonstrated positive reaction within the whole tissue.
Indirect immunofluorescence test on normal monkey retina
revealed in 11 patients the presence of other
autoantibodies; antinuclear and antinucleolar antibodies,
reacting with nuclei of inner and outer nuclear layers
of retina.
Conclusions: The presence of ARA in serum of
patients with choroidal melanoma before therapy may be a
result of autoimmune reaction against the tumor tissue.
The detection of ARA in serum after plaque therapy may
be associated with reaction due to tumor irradiation.
The appearance of other serum autoantibodies in patients
with choroidal melanoma may indicate at more advanced
and generalized process. |
| Słowa kluczowe: |
przeciwciała
przeciwsiatkówkowe, immunofluorescencja pośrednia,
czerniak naczyniówki. |
| Key words: |
antiretinal antibodies,
indirect immunofluorescence, choroidal melanoma. |
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