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NR 7-9/2006
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Zastosowanie metody
łańcuchowej reakcji polimerazy (PCR) w diagnostyce zakażeń
wnętrza gałki ocznej
Application of polymerase
chain reaction method (PCR) in diagnosis of endophthalmitis
Marek Gerkowicz1, Małgorzata
Pietraś-Trzpiel1, Maria Kozioł-Montewka2,
Ewa Kosior-Jarecka2, Małgorzata Latalska1,
Jolanta Paluch-Oleś2
1 Z II Kliniki Okulistyki Akademii Medycznej w
Lublinie
Kierownik: prof. dr hab. n. med. Marek Gerkowicz
2 Z Katedry i Zakładu Mikrobiologii Lekarskiej
Akademii Medycznej w Lublinie
Kierownik: prof. dr hab. n. med. Maria Kozioł-Montewka |
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| Summary: |
Purpose: The goal
of this study is to determine the usefulness of the PCR
method in the diagnosis of endophthalmitis.
Materials and methods: 30 clinical specimens 18
AH and 12 VF were obtained from 20 eyes with the
clinical diagnosis of endophthalmitis. These included:
14 cases after cataract surgery, 1 case post
trabeculectomy, 2 cases after penetrating traumas, and 3
cases after endogenous endophthalmitis. The same samples
were analysed using 2 different methods: 1. conventional
microbiological techniques (microscopy and diagnostic
culture) and 2. PCR directed at 16S rDNA using universal
primers.
Results: In the aqueous humor the causative
pathogen was identified in one case (5,2%) by using
diagnostic culture compared with seven cases (39%) by
using PCR methods. In the vitreous samples the pathogen
was identified in one case (9%) by using conventional
method compared with five cases (50%) by using PCR.
Microscopic preparation was difficult to evaluate in all
samples.
Conclusions: PCR performed on aqueous humor and
vitreous fluid is a reliable tool for diagnosis of
causative organism particularly in smear and culture
negative specimens. By using universal primers we are
able to detect the presence of pathogen in case of
endophthalmitis and than potentially by using DNA probe
hybridization to determine the species of the bacteria.
The discrimination between infection or non-infection
endophthalmitis plays the main role in a succesful
therapy. |
| Słowa kluczowe: |
zakażenia wnętrza gałki
ocznej, PCR. |
| Key words: |
endophthalmitis, PCR. |
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