NR 5-6/2002
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Ocena komórek nabłonka soczewki w mikroskopie świetlnym
i elektronowym oraz metodami immunohistochemicznymi w zaćmach różnego typu
A study of human lens epithelial cells by light and electron
microscopy and by immunohistochemistry in different types of cataracts
Aleksandra Synder1, Aleksandra
Omulecka2,
Marzena Ratyńska3, Wojciech
Omulecki1
1Z Katedry i Kliniki Chorób Oczu Akademii Medycznej w Łodzi
Kierownik: dr hab. n. med. Wojciech Omulecki
2Z Katedry i Zakładu Patomorfologii Akademii Medycznej w Łodzi
Kierownik: prof. dr hab. n. med. Wielisław Papierz
3Z Samodzielnej Pracowni Mikroskopii Elektronowej Katedry Patomorfologii Akademii Medycznej w Łodzi
Kierownik: prof. dr hab. n. med. Michał Karasek |
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| Summary: |
Purpose: To evaluate the characteristics of cataract changes in lens epithelial cells (LECs), in different types of human cataract.
Material and methods: Anterior capsules for the study were obtained from patients with different types of cataracts during extracapsular cataract extraction, or phacoemulsification using continuous curvilinear capsulorhexis. LECs attached to the anterior capsules were analyzed for morphological changes by light and electron microscopy, and for cellular characteristics by immunohistochemistry. The reactivity to cytokeratins 5,6,8,17 and 19 (arker for epithelial cells) and to vimentin (arker for mesenchymal cells) was determined.
Results: The consecutive degenerative changes were observed in most of the cells: multilayered cells, nuclei of abnormal diameters and shapes, vacuolation of nuclei and cytoplasm. LECs were immunohistochemically positive for cytokeratin and vimentin, or only for vimentin in all types of cataract. Some of LECs showed morphological and immunohistochemical characteristics of mesenchymal cells.
Conclusions: Lens epithelial cells show simmilar degenerative changes in different types of cataract and may have the ability to transdifferentiate into mesenchymal
cells. |
| Słowa kluczowe: |
komórki nabłonka soczewki, zaćma, mikroskopia świetlna, mikroskopia elektronowa, immunohistochemia. |
| Key words: |
lens epithelial cells, cataract, light microscopy, transmission electron microscopy, immunohistochemistry. |
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